Problems in DNA replication may lead to breaks in the replication fork, and recombinational reactions occur to restore the integrity of the fork via strand-invasion of the broken chromosome with its homologous strand.
If this happens within repeated DNA sequences, genetic rearrangements may be produced.
The bacterial UmuC/DinB family consists of bypass polymerases that are responsible for translesion DNA synthesis.
DinB, also referred to as DNA polyermase IV or DNA polymerase k, is an SOS-inducible, error-prone DNA polymerase that plays a role in DNA damage-induced mutagenesis by preferentially making frameshift mutations.
DinB is uniquely and highly expressed in the adrenal cortex and testis, as well as in a variety of other tissues.
p53 regulates DinB and exposure to various DNA-damaging agents causes an upregulation of DinB.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of POLK. Species Crossreactivity: Human, mouse, rat
