The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing.
The gene encoding human PML maps to chromosome 15q22.
The t(15;17) (q22;q11.2-q12) chromosomal trans-location of the retinoic acid receptor alpha (RARalpha) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARalpha chimeric protein.
Myeloid precursor cells expressing the PML/ RARalpha chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis.
PML/RARalpha transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation.
PML/ RARalpha also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PML. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to the N-terminal of Human PML.
