PLK1 (polo-like kinase 1) is a member of the serine/threonine protein kinase family, cdc5/polo subfamily.
PLK1 contains two polo box domains with a predicted molecular weight of 68 kDa.
PLK1 has been shown to regulate cdc2/cyclin B through phosphorylation and activation of cdc25c phosphatase.
PLK1 is modified by phosphorylation at Threonine 210.
PLK1 may also be required for cell division.
Depletion of PLK1 results in apoptosis and deregulation of expression of PKL1 is correlated with development of many malignancies.
Studies have shown that ab14209 preferentially detects the kinetochore-associated pool of Plk1.
An alternative antibody, Mouse monoclonal to Plk1 (ab14210) preferentially stains centrosomal-associated Plk1.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PLK1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 180-220 of Human PLK1.
