Activation of protein kinase C (PKC) in platelets results in immediate phosphorylation of pleckstrin (previously called 40K or P47), the major PKC substrate in platelets.
Pleckstrin contains a Ca2+-binding ‘EF-hand’ structure and PKC phosphorylation sites at Ser-113 and Ser-117.
The N and C termini of pleckstrin contain two pleckstrin homology domains (PH), which mediate protein-protein and protein-lipid interactions.
Pleckstrin is highly expressed in human neutrophils.
Pleckstrin is rapidly phosphorylated following treatment of neutrophils in response to inflammatory stimuli, probably by nonconventional PKC isoforms delta or zeta, which are expressed in human neutrophils.
Phosphorylation by nonconventional PKC isoforms induces a conformational change in pleckstrin that promotes its interaction with membranes and/or with the cytoskeleton, serving to target proteins or lipids recognized by PH domains to sites where they can contribute to the microbicidal response.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PLEK. Species Crossreactivity: Human, mouse
