Phosphatidylcholine phospholipase D1 and D2 (PC-PLD1 and PC-PLD2) are phospholipid-specific phosphodiesterases that hydrolyze phosphatidylcholine.
Unlike PC-PLD1, which associates with secretory granules, PC-PLD2 localizes to the plasma membrane, where it is implicated in the formation of endocytotic vesicles.
Both PC-PLD1 and PC-PLD2 coordinately regulate macrophage phagocytosis.
PC-PLD activity in mammalian cells is transiently stimulated upon activation by G protein-coupled and receptor tyrosine kinase cell surface receptors.
In addition, tubulin binding to PC-PLD2 inhibits muscarinic receptor-linked PC-PLD2 activation.
PC-PLD2 also enhances PKCzeta activity through direct interaction in a lipase activity-independent manner.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PLD2, phosphorylated Y169. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human PLD2 around the phosphorylation site of Tyrosine 169.
