Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptormediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol fromphosphatidylinositol 4,5-bisphosphate.There are manymammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC y1, PLC y2, PLC delta1, PLC delta2 and PLC ε.
After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC y2 associateswith several tyrosine-phosphorylated proteins(Syk, SLP-76,Lyn, linker for activation of T cells(LAT) and the FcR y chain),which bind to its C-terminal SH2 domain.
PLC y1 associateswith Syk in B cells, but PLC y2 does not associatewith Sykin platelets.
The C-terminal SH2 domain isinvolved in the regulation of PLC y2.
In addition, Btkcan induce PLC y2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid PBS, pH 7.2, 0.05% sodium azide, 50% glycerol
Specificity：Recognizes endogenous levels of human PLC γ2, phosphorylated Y759. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to residues in human PLC γ2 around the phosphorylation site of Tyrosine 759.
