Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC y1, PLC y2, PLC ∂1, PLC ∂2 and PLC e.
After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC y2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR y chain), which bind to its C-terminal SH2 domain.
PLC y1 associates with Syk in B cells, but PLC y2 does not associate with Syk in platelets.
The C-terminal SH2 domain is involved in the regulation of PLC y2.
In addition, Btk can induce PLC y2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence .
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PLC γ2. Species Crossreactivity: mouse
