Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.
There are many mammalian PLC isozymes, including PLC beta1, PLC beta2, PLC beta3, PLC beta4, PLC y1, PLC y2, PLC delta1,PLC delta2 and PLC ε).
PLC betas are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors.
Two alternatively spliced forms (1,181 and 1,166 amino acids) of PLC beta2 are generated in hematopoietic cells that differ in the carboxyl-terminal sequence implicated in interaction of PLC beta enzymes with Galphaq.The pleckstrin homology domain of PLC beta2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory efect on basal PLC beta2 activity.
PLC beta2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PLC beta2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 382-427 of Human PLC β2.
