PREP-2 (Pbx-regulating protein-2), also known as PBX/knotted 1 homeobox 2 or PKNOX2, is a widely expressed protein belonging to the TALE (three amino acid loop extension)/MEIS family.
PREP-2 is a DNA-binding protein that forms stable complexes with Pbx proteins.
It is highly homologous to the related protein PREP-1, but displays a more restricted tissue distribution and a higher DNA-dissociation rate.
Like PREP-1, PREP-2 forms a heterodimer with Pbx 1.
The PREP-2-Pbx 1 dimer is relocated to the nucleus where it associates with HoxB1 to form a ternary complex.
In contrast with PREP-1, which acts to increase transcriptional activation in this ternary complex, PREP-2 leads to a slight decrease in transcriptional activity of the ternary complex.
Multiple isoforms exist for PREP-2, localizing to the nucleus or cytoplasm.
Cytoplasmic isoforms are believed to colocalize with F-actin, G-actin and tubulin/microtubules.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PKNOX2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 330-378 of Human PKNOX2.
