The PKM2 (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events.
Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate.
In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1.
The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 500-1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PKM2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 200-250 of Human PKM2.
