In mammals, four different isoenzymes exist for pyruvate kinase.
Based on their tissue distribution, the isoenzymes are designated L-type (for predominant expression in the liver), R-type (for predominant expression in red blood cells), M1-type (for predominant expression in muscle, brain and heart) and M2-type (for predominant expression in fetal tissues).
Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP.
The PKLR (pyruvate kinase, liver and RBC) gene encodes the L- and R-type isoenzymes through alternative splicing events under the control of different promoters.
The R-type isoform, also known as RPK (R-type pyruvate kinase), exists as a tetramer and, when functioning improperly, can result in chronic/hereditary nonspherocytic hemolytic anemia (CNSHA/HNSHA) or pyruvate kinase hyperactivity (also called high red cell ATP syndrome).
The L-type isoform, alternatively known as PKL (pyruvate kinase L-type), also exists as a tetramer and is upregulated by glucose with implications in maturity-onset diabetes of the young (MODy).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PKLR. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human PKLR.
