Members of the protein kinase C (PKC) family play a key regulatory role in variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function.
PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids.
Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC.
PKCs can be subdivided into at least two major classes including conventional (c) PKC isoforms (alpha, betaI, betaII and y) and novel (n) PKC isoforms (delta, ε , zeta, η and θ).
Patterns of expression for each PKC isoform differs among tissues and PKC family members exhibit clear differences in their cofactor dependencies.
For instance, the kinase activities of nPKC delta and ε are independent of Ca++.
On the other hand, nPKC and ε, as well as all of the cPKC members, possess phorbol ester-binding activities and kinase activities.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PKC θ. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 520-570 of Human PKC θ.
