The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA).
Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme, resulting in release of active catalytic subunits.
Four different PKA regulatory subunits have been identified, designated Ia, Ib, IIa and IIb.
The PKA Ia reg protein is a tissue-specific extinguisher that downregulates the expression of seven liver genes in hepatoma x fibroblast hybrids.
Functional null mutations in the gene that codes for PKA Ia reg cause Carney complex (CNC).
CNC is an autosomal dominant multiple neoplasia syndrome.
CNC is associated with a variety of characterized symptoms such as cardiac and other myxomas, spotty skin pigmentation, endocrine tumors and psammomatous melanotic schwannomas.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PKA Ialpha reg. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant full length Human PKA Iα reg.
