NIMA was originally shown in Aspergillus nidulans to be necessary for entry into mitosis.
NIMA-related mammalian proteins have since been identified as Nek1, Nek2 and Nek3.
High expression of Nek1 is seen in male and female germ cell lines of mouse.
Nek2 is the closest known mammalian relative to NIMA.
Like NIMA, Nek2 expression peaks at the G2 to M phase transition.
Pin1 was originally identified as a NIMA-interacting protein.
Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase), which specifically binds to phosphoserine-proline or phosphothreonine-proline bonds in mitotic phosphoproteins.
While previously identified PPIases have been shown to be involved in protein folding, assembly and transport, Pin1 is the first PPIase to be identified as a required protein for cell viability.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PIN1, phosphorylated S16. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from human PIN1 around the phosphorylation site of Serine 16.
