Phosphatidylinositol-glycans (PIGs) are multi-pass transmembrane proteins that localize to the endoplasmic reticulum.
PIGs exhibit various functions, but all are crucial for the biosynthesis of the glycosylphosphatidylinositol (GPI)-anchor.
Some PIG proteins are components of the GPI transamidase complex and play a role in the recognition of either the GPI attachment signal or the lipid portion of GPI.
Other PIGs belong to the glycosyltransferase complex and function in the transfer of N-acetylglucosamine (GlcNAc) to phosphatidylinositol (PI).
A variety of other PIGs play distinct roles in GPI synthesis including mannosylation of the GPI-anchor.
PIG-W (Phosphatidylinositol-glycan biosynthesis class W protein) is a 504 amino acid multi-pass membrane protein that functions in the third step of GPI biosynthesis and acylates the inositol ring of phosphatidylinositol.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PIGW. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human PIGW.
