84-2380-45　［取扱停止］PIG3 (Quinone oxidoreductase PIG3, Tumor Protein p53-inducible Protein 3, p53-induced Gene 3 Protein, TP53I3, PIG3) 50ul　225036

特徴

• The PIG (p53-induced gene) gene family encodes redox-controlling proteins that are involved in p53 tumor suppressor activity.
• One member of the PIG gene family, p53-inducible gene 3 (PIG3), is a p53 responsive gene that maps, in humans, to chromosome 2p and encodes a protein with significant homology to oxidoreductases.
• Oxidoreductases are enzymes involved in cellular responses to oxidative stress and irradiation, and they influence the involvement of PIG3 in the metabolism of reactive oxygen species.
• PIG3 is localized to the cytoplasm and induced in primary, non-transformed, and transformed cell cultures after exposure to genotoxic agents.
• The induction of PIG3 is p53 dependent and occurs with delayed kinetics as compared with other p53 downstream targets.
• PIG3 may act with caspase-8 as a key regulatory element in p53-dependent transcriptional deregulation by triggering the caspase cascade and mitochondrial breakdown.
• PIG3 is highly up-regulated by p53 and may be useful for detecting transient activation of p53.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.