84-2380-41　［取扱停止］PIF1 (ATP-Dependent DNA Helicase PIF1, DNA Repair and recombination Helicase PIF-1, PIF1/RRM3 DNA Helicase-like Protein, C15orf20) 50ul　225034

特徴

• PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first.
• This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae.
• The PIF1 gene is highly conserved from yeast to humans.
• Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation.
• Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity.
• PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.