84-2379-85　［取扱停止］PHLPP1 (PH Domain Leucine-Rich Repeat-Containing Protein Phosphatase 1, Pleckstrin Homology Domain-Containing Family E Member 1, PH Domain-Containing Family E Member 1, Suprachiasmatic nucleus circadian oscillatory Protein, hSCOP, PHLPP1, KIAA0606, PHLPP, PLEKHE1, SCOP) 100ul　225005

特徴

• SCOP (Suprachiasmatic nucleus circadian oscillatory protein, PHLPP, PH domain and leucine rich repeat protein phosphatase, PLEKHE1) is a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth.
• Endogenous SCOP in human embryonic kidney cell lysates produces a major protein and minor protein.
• SCOP negatively regulates K-Ras signaling in membrane rafts and contributes to the regulation of the Ras-MAPK signaling pathway.
• Recombinant SCOP can dephosphorylate the hydrophobic motif of Akt1 (ser-473) in vitro, triggering apoptosis and suppressing tumor growth.
• SCOP levels appear lower in certain colon cancer and glioblastoma cell lines that show elevated Akt phosphorylation.
• Rat tissues that express SCOP include cerebrum, cerebellum, and testis.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.