PHF3 (PHD finger protein 3) is a 2039 amino acid ubiquitously expressed protein that is phosphorylated upon DNA damage by either ATM or ATR.
PHF3 contains a PHD finger motif, a proline-rich region, a TFIIS domain and two bipartite nuclear localization signals.
Since these motifs are frequently found in proteins involved in transcription, PHF3 may function as a transcription factor.
Expression of PHF3 is significantly reduced in anaplastic astrocytomas, glioblastomas and glioblastoma cell lines, suggesting that PHF3 plays a role as a tumor suppressor.
Antibodies against PHF3 are present in some patients afflicted with glioblastoma multiforme and presence of the , correlates to significantly better survival rates.
There are two isoforms of PHF3 that are produced as a result of alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PHF3. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to the C-terminus of Human PHF3.
