Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression.
PHF21A (PHD finger protein 21A), also known as BRAF35-HDAC complex protein BHC80, is a 680 amino acid nuclear protein that contains one PHD-type zinc finger and one A.T hook DNA-binding domain, suggesting involvement in transcriptional regulation events.
PHF21A is a component of the BHC complex, which is responsible for repressing transcription of neuron-specific genes in non-neuronal cells.
The BHC complex acts as a chromatin modifier that deacetylates and demethylates specific sites on histones.
PHF21A may act as a scaffold within the BHC complex.
Predominantly expressed in brain, three isoforms of PHF21A exist as a result of alternative splicing events.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PHF21A. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to full length human PHF21A.
