PHF1 (PHD protein finger 1), also known as polycomb-like protein 1 or hPCI1, is a 567 amino acid member of the PHD finger protein family.
Members of the PHD finger protein family function as transcriptional regulators that affect gene expression by modulating chromatin structure.
With a subcellular localization to the nucleus, PHF1 is widely expressed in tissues, with high expression in pancreas, heart and skeletal muscle and low expression in liver, lung, kidney, brain and placenta.
PHF1 contains 2 PHD-type zinc finger domains, which may contribute to the transcriptional activity of PHF1.
PHF1 is thought to interact with ENX-1, a component of PRC2 (polycomb repressive complex 2), increasing the gene silencing activity of PRC2.
PHF1 exists as two isoforms produced by alternative splicing.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PHF1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 361-410 of Human PHF1.
