84-2379-67　［取扱停止］PHC3 (Polyhomeotic-like Protein 3, Early Development Regulatory Protein 3, Homolog of polyhomeotic 3, hPH3, PHC3, EDR3, PH3) 100ul　224996

特徴

• Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development.
• PHC3 (Polyhomeotic-like protein 3), also known as Early development regulatory protein 3, is a 983 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex.
• Specifically, the PcG PRC1 complex modifies histones, remodels chromatin and mediates monoubiquination of Histone H2A.
• Other constituent proteins involved in the PcG PRC1 complex are Mel-18, Bmi-1, M33, MPc2, MPc3, RING1, Ring1b, as well as several others.
• Loss of heterozygosity of the PHC3 gene was observed in human osteosarcoma tumors, suggesting that loss of PHC3 protein may contribute to tumorigenesis.
• There are six isoforms of PHC3 that are expressed as a result of alternative splicing events.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.