Phosphoglucomutase, which belongs to the phophohexose mutase family, plays a role in glycogen catabolism (glycogenolysis) as well as in the process of glycogen synthesis (glycogenesis).
During glycogenolysis, PGM converts glucose-1-phosphate to glucose-6-phosphate, thus promoting glycolysis and the pentose phosphate pathway.
During glycogenesis, PGM functions in the opposite manner, converting glucose-6-phosphate into glucose-1-phosphate, to facilitate glycogen synthesis.
PGM has three structural loci: PGM1, PGM2 and PGM3.
These three genetic forms of PGM differ in amino acid sequences but catalyze the same reactions, therefore indicating that they are isozymes.
PGM1, a 562 amino acid protein, is highly polymorphic; three mutations and four intragenic recombination events between the three mutation sites generate eight protein variants.
All phosphoglucomutases act as monomers and bind one magnesium ion per subunit.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PGM1. Species Crossreactivity: Human, mouse, rat
