Biological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors.
The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered.
A number of transcription factors appearing to be molecular components of the SCN clock have been identified.
Mutations within the Clock gene increase the length of the endogenous period and cause a loss of rhythmicity of circadian oscillations.
Three mammalian period proteins, designated Per1, Per2 and Per3, exhibit circadian rhythyms in the SCN.During subjective night, Per1 and Per2 RNA levels increase in response to light pulses while Per3 RNA levels show no change in response to light pulses.
Tim, for timeless, interacts with Per1 as well as Per2; and Tim and Per1 negatively regulate Clock-BMAL1-induced transcription.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of Per3. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids N-terminus of Human Per3.
