Pigment epithelium derived factor, originally identified in conditioned medium of cultured human fetal retinal pigment epithelial (RPE) cells, is a neurotrophic protein that induces extensive neuronal differentiation in human y79 retinoblastoma cells, a neoplastic counterpart of normal retinoblasts.
It has been suggested that PEDF is synthesized by RPE cells and secreted into the retina interphotoreceptor matrix where it may influence development/differentiation of the neural retina.
PEDF is a potent inhibitor of angiogenesis.
As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
The PEDF gene is a member of the serpin gene family.
Serpins are a group of serine protease inhibitors, some of which have also been reported to exhibit neurotrophic activity.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PEDF. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 280-320 of Human PEDF.
