Raf kinase inhibitor protein (RKIP) is a cytosolic protein that was initially characterized as a phosphatidylethanolamine-binding protein (PBP) expressed in brain tissue and secreted from testis fluid.
In addition, RKIP was identified by yeast two-hybrid screening of human T cell libraries directed at identifying proteins that associate with the BXB kinase domain of the serine/threonine kinase, Raf-1.
Subsequent in vitro and in vivo studies indicate that RKIP binds to both the active and inactive forms of Raf-1 and thereby regulates the signaling cascade of the MAP kinase pathway.
The specific association of RKIP with kinase-active Raf-1 competitively inhibits the binding and activation of the Raf-1 substrate MEK.
RKIP, in turn, affects downstream MAP kinase signaling by decreasing the activation of MEK effector proteins, including ERK1 and ERK2, and the subsequent induction of AP-1 mediated transcription.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PEBP1. Species Crossreactivity: Human, mouse, rat
