PDLIM5 (PDZ and LIM domain 5), also known as L9, ENH (Enigma homolog),LIM or ENH1, is a member of the Enigma family of proteins.
Proteins belonging to this family contain an N-terminal PDZ (post-synaptic density-95/discs large/zone occludens-1) domain and one to three C-terminal LIM domains that typically associate with various isoforms of PKC (protein kinase C).
Expressed in a wide variety of tissues, PDLIM5 contains three LIM zinc-binding domains and one PDZ domain.
In the brain, PDLIM5 colocalizes with synaptic vesicles of neurotransmitters and regulates neuronal calcium signaling through an interaction with PKC ε and N-type Ca++ CP alpha1B (N-type calcium channel alpha1B subunit).
Expression of PDLIM5 is often increased in the brain of patients with schizophrenia, major depression and bipolar disorder.
This suggests that PDLIM5 may contribute to the genetic susceptibility of such conditions.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PDLIM5, phosphorylated Y251. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from human PDLIM5 around the phosphorylation site of Tyrosine 251.
