ALP (actinin-associated LIM protein), also known as PDLIM3 (PDZ and LIM domain protein 3) is a 364 amino acid protein that contains one LIM zinc-binding domain and one PDZ domain and localizes to the cytoplasm, as well as to myofiber Z-lines.
Existing as three alternatively spliced isoforms, two of which exhibit tissue-specific expression in skeletal muscle and heart, ALP interacts with å-actinin-2 and, via this interaction, is thought to play a role in Actin filament organization, specifically regulating the association of Actin filaments arrays with muscle cells.
The gene encoding ALP maps to a region on human chromosome 4 that is associated with facioscapulohumeral muscular dystrophy, suggesting that defects in the ALP gene may be involved in the pathogenesis of muscular dystrophy.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PDLIM3. Species Crossreactivity: Human, mouse
