PDGF is a mitogen for mesenchyme- and glia-derived cells.
It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF-AA, PDGF-AB and PDGF-BB.
The three isoforms bind with different affinities to two receptor types, alpha and beta, which are structurally related and endowed with protein-tyrosine kinase domains.
Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to alpha receptors with high affinity, whereas the B subunit can bind to both alpha and beta receptors.
Evidence suggests that PDGF may function as a neurotrophic factor.
The fact that receptors for PDGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PDGF-A. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 121-160 of human PDGF-A.
