cAMP-hydrolyzing cyclic nucleotide phosphodiesterase (PDE) catalyzes hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates.
PDEs are key enzymes in signaling pathways that influence smooth muscle tone regulation.
The PDE1 family are calmodulin-dependent (CaM-PDEs) that undergo stimulation through a calcium-calmodulin complex.
Human PDE1B (PDE1B1) protein is present in neuronal cells of the cerebellum, hippocampus, and caudate, and lymphoblastoid lines, such as RPMI-8392 cells.
PDE1B may participate in learning, memory, and regulation of phosphorylation of DARPP-32 in dopaminergic neurons.
A splice variant known as PDE1B2 encodes a 516-amino acid protein and diverges from PDE1B1 by the replacement of the first 38 residues with an alternative 18 residues.
The human PDE1B gene maps to chromosome 12q13, contains 13 exons, and encodes a 536 amino acid protein.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of PDE1B. Species Crossreactivity: Human, mouse, rat
