Pyruvate carboxylase (PCB) is a biotinylated mitchondrial enzyme that catalyzes the synthesis of oxaloacetate from pyruvate in a tissue specific manner.
In addition to covalently binding the biotin cofactor, PCB contains consensus sequences for the attachment of ATP and the substrate pyruvate.
The PCB gene is located on the long arm of chromosome 11.
Mutations in PCB metabolism (pyruvate carboxylase deficiency) are known to cause lactic acidosis, hypoglycemia and mental retardation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PCB. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 400-445 of Human PCB.
