Activation of phosphatidylcholine-specific phospholipase D (PC-PLD) catalyzes the hydrolysis of phosphatidylcholine (PC) to generate phosphatidic acid (PA).
Insulin activates the PLD-dependent hydrolysis of PC in plasma membranes of adipocytes by a mechanism that may involve wortmannin-sensitive phosphatidylinositol 3-kinase.
In addition to the transient activation by growth factors stimulation, PC-PLD is constitutively activated in some of the Src- and Ras-transformed cells.
PC-PLD is one of the target enzymes of ischemia; its decrease may cause a perturbation of PC hydrolysis and/or disorders of intracellular signal transduction or choline metabolism for acetylcholine formation in the brain.
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PC-PLD1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 524-573 of Human PC-PLD1.
