Pax-4 (paired box gene 4) protein influences normal differentiation of insulin-producing beta cells and influences normal pancreatic islet development.
Pax-4 protein is a transcriptional repressor that binds to a common cis element in the Glucagon, insulin and Somatostatin promoters.
Mouse Pax-4 transcript is present in pancreatic islets, and the islet beta cell lines MIN6, beta TC, and NIT1.
Differentiation of endoderm-derived endocrine pancreas is mediated through Pax-4 and Pax-6.
Pax-4 may act as a Pax-6 repressor due to the competition for binding sites and lower transactivation potential of Pax-4.
The human Pax-4 gene encodes a deduced 350 amino acid protein that is 80% identical to the deduced mouse Pax-4 protein.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of human PAX4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to full length human PAX4. Swiss/UniProt Accession: O43316
