Pax genes contain paired domains that share strong homology to genes in Drosophila which are involved in programming early development.
The product of the PAX3 gene is a DNA-binding protein expressed during early neurogenesis.
Pax-3 is a protein containing both a paired domain and a paired-type homeodomain.
During early neurogenesis, Pax-3 expression is limited to mitotic cells in the ventricular zone of the developing spinal cord and to distinct regions in the hindbrain, midbrain and diencephalon.
In 10-12 day embryos, expression of Pax-3 is also seen in neural crest cells of the developing spinal ganglia, the craniofacial mesectoderm and in limb mesenchyme.
Mutations in the MITF and Pax-3 genes, encoding transcription factors, are responsible for Waardenburg syndrome II (WSII) and WSI/WSIII, respectively.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Pax-3. Species Crossreactivity: Human, mouse, rat
