84-2377-33　［取扱停止］PARP, Cleaved (PARP1, ADPRT, PPOL, ADPRT 1, ARTD1, Poly [ADP-ribose] Polymerase 1, ADP-ribosyltransferase diphtheria toxin-like 1) 100ul　224877

特徴

• PARP, a 116kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress.
• This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo.
• In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa).
• PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.