The DJ-1 gene encodes a highly-conserved protein which is implicated in a number of cell processes.
DJ-1 was first identified as a novel oncogene that transformed mouse NIH/3T3 cells in cooperation with activated Ras.
Additionally, DJ-1 was cloned in rat as SP22 or CAP-1, and found to be an infertilityrelated sperm protein whose expression is reduced in sperm treated with toxicants.
DJ-1 also positively regulates the androgen receptor (AR) by forming a complex with PIASxalpha, a negative regulator of AR.
The gene encoding human DJ-1 maps to chromosome 1p36.33-p36.12, a region identified as a hot spot of chromosome abnormalities in several tumor cells.
Subsequently, mutations in the DJ-1 gene have been implicated in Parkinson’s disease, and loss of DJ-1 function leads to neurodegeneration.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of PARK7. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 50-97 of Human PARK7 / DJ-1.
