84-2376-52　［取扱停止］p73, phosphorylated (Y99) (p73, p53-like Transcription Factor, p53-Related Protein, p73, P73) 100ul　224837

特徴

• p73 protein is a member of the p53 family of proteins.
• The tumor-suppressor protein p53 exhibits sequence specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage.
• In response to signals generated by a variety of genotoxic stresses, e.g, UV irradiation or DNA damage, p53 is expressed and undergoes post translational modification that results in its accumulation in the nucleus.
• Activation of p53 leads to cell cycle arrest and in some cases to apoptosis, resulting in the inability of genetically damaged cells to proliferate.
• Thus, the p53 dependent pathways help to maintain genomic stability by eliminating damaged cells.
• The accumulation of high levels of p53 is a potential marker for malignancy.
• p73 protein is expressed in either full length form (p73 alpha 80 kDa) or a shorter (p73 beta 70 kDa) mRNA variant.
• p73 gene was predicted to encode a protein with significant amino acid sequence similarity to p53.
• Each of the p53 amino acid residues implicated in direct sequence specific DNA binding is conserved in p73.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.