p53 is a transcription factor that mediates cell cycle arrest and apoptosis by binding to DNA and activating the transcription of specific genes.
p53 is also thought to be involved in DNA repair by the transcriptional activation of a ribonucleotide reductase gene, p53R2, after exposure to genotoxic stresses.
p53R2 displays a significant similarity to ribonucleotide reductase small subunit (R2), and the expression of R2 is elevated at the onset of the S-phase of the cell cycle.
However, only p53R2 expression is induced in response to ultraviolet and g-irradiation and adriamycin treatment.
p53R2 translocates to the nucleus upon DNA damage, and subsequently, supplies an immediate pool of dNTPs necessary for DNA repair.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of p53R2. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 123-178 of Human p53R2.
