The EF-hand domain is a twelve amino acid loop motif that is commonly found in proteins that participate in calcium-binding events within the cell.
EF-hand domains generally exist in a pair that, together, form a stable four-helix bundle that enables the binding of calcium ions.
PH-4, also known as P4HTM (prolyl 4-hydroxylase, transmembrane), PHD4 or EGLN4, is a 502 amino acid single-pass type II membrane protein that localizes to the endoplasmic reticulum and contains one PKHD domain and two EF-hand domains.
Expressed in a variety of tissues with highest expression in heart, brain, kidney, placenta and skeletal muscle, PH-4 uses iron as a cofactor to catalyze the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins, thereby playing an important role in hypoxia adaptation and cellular oxygen sensing.
Multiple isoforms of PH-4 exist due to alternative splicing events.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of P4HTM. Species Crossreactivity: Human, mouse
