Oncostatin M (OSM) is a glycoprotein that was originally isolated from the conditioned medium of U937 human histiocytic leukemia cells that had been induced to differentiate into macrophage-like cells by treatment with phorbol 12-myristate 13 acetate.
OSM inhibits the growth of a broad range of human tumor cell lines, but does not influence the growth of normal human fibroblasts.
High-affinity binding sites for OSM have been detected on normal and tumor cells, and a receptor has been identified by chemical cross-linking studies.
Expression of OSM is greatest in activated monocytic and lymphocytic cell lines and in normal adherent macrophages.
Amino acid sequence analysis of OSM has revealed homology with leukemia inhibitory factor (LIF), granulocyte colony stimulating factor (G-CSF) and interleukin 6 (IL-6), all of which affect the growth and differentiation of a broad range of cell types, including those of hematopoietic origin.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of OSM. Species Crossreactivity: Human
