The first such recessive obesity mutation, the obese mutation (Ob), was identified in 1950.
Mutation of Ob results in profound obesity and type II diabetes as part of a syndrome that resembles morbid obesity in humans.
It has been postulated that the Ob gene product may function as a component of a signaling pathway in adipose tissue that functions to regulate body fat depot size.
The cloning and sequence analysis of the mouse Ob gene and its human homolog has recently been described.
Ob encodes an adipose tissue-specific mRNA with a highly conserved 167 amino acid open reading frame.
The predicted amino acid sequence is 84% identical between human and mouse and has the features of a secreted protein.
A nonsense mutation in codon 105 has been found in the original congenic C57BL/6J Ob/Ob mouse strain.
The Ob gene encodes the protein leptin.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, pH 7.2, 0.02% sodium azide, 50% glyceol.
Specificity：Recognizes endogenous levels of Ob-R. Species Crossreactivity: Human
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to amino acids 650-700 of Human Ob-R.
