The cadherins are a family of Ca++-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis.
Cadherins each contain a large extracellular domain at the amino terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity.
The relatively short carboxy terminal, intracellular domain interacts with a variety of cytoplasmic proteins, including catenin beta, to regulate cadherin function.
Two forms of OB-cadherin (for osteoblast-cadherin, also designated cadherin-11 or OSF-4) have been identified as OBcadherin- 1 and OB-cadherin-2.
Both OB-cadherins are expressed in osteoblastic cell lines and low expression is also seen in lungs, testis and brain.
OB-cadherin-2 has a truncated cytoplasmic domain.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of OB-cadherin. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 430-480 of Human OB-cadherin.
