MTH1, also known as NUDT1 (nudix (nucleoside diphosphate linked moiety X)-type motif 1), is a 179 amino acid cytoplasmic protein that is a member of the nudix hydrolase family.
Highly expressed in testis, thymus and proliferating blood lymphocytes, MTH1 functions as an antimutagenic that hydrolyzes oxidized purine nucleoside triphosphates to their corresponding monophosphates.
Through its ability to enzymatically hydrolyze ATP and GTP to AMP and GMP, respectively, MTH1 prevents misincorporation of GTP into DNA, thus preventing A:T to C:G transversions.
The cytoplasmic location of MTH1, along with its antimutagenic capabilities, suggests that it may also be involved in the sanitization of nucleotide pools for both mitochondrial and nuclear genomes.
Four isoforms of MTH1 exist—three of which are formed due to alternative splicing events and one of which is formed via a single-nucleotide polymorphism.
Overexpression of MTH1 is implicated in prostate and cell lung carcinomas.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NUDT1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：A synthetic peptide corresponding to residues in Human NUDT1.
