Nuclear respiratory factor-1 (NRF-1) is a transcriptional activator that has been implicated in the nuclear control of respiratory chain expression in mammalian cells.
The NRF-1 gene is expressed during oogenesis and during the early stages of embryogenesis.
In vitro studies have implicated NRF-1 in the transcriptional expression of nuclear genes required for mitochondrial respiratory function, as well as for other fundamental cellular activities.
While most isolated wild-type and NRF-1+/- blastocysts continue to develop normally in vitro, NRF-1-/- blastocysts lack this ability, despite their normal morphology.
NRF-1 is specifically required in the maintenance of mtDNA and respiratory chain function during early embryogenesis.
NRF-1 also plays a key role in cellular adaptation to energy demands by translating physiological signals into an increased capacity for generating energy.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NRF-1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 224-276 of Human NRF-1.
