NPy2-R is a member of the Gprotein-coupled receptor super-family, and like other members has seven putative transmembrane domains.
However, NPy2-R gene consists of two exons, unlike the contiguous structure of other G-protein-coupled receptor genes.
NPy2-R shares a 31% sequence identity with NPy1-R.
NPy2-R is expressed in the presynaptic membranes of the central nervous system, and to a lesser extent the peripheral nervous system.
NPy2-R associates (from highest to lowest affinity) with carboxyl terminals of unphosphorylate Peptide yy (Pyy) (phosphorylation of Pyy greatly reduces its binding affinity),NPy, and Pancreatic Peptide.
Depending on the cell type, NPy2-R couples with different G proteins, which act as different second messengers.
NPy2-R reduces the effects of depolarization, calcium ion currents in arterial smooth muscle by through association with NPy.
Applications:Suitable for use in Immunofluorescence, Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 15mM sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NPY2-R. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 30-80 of Human NPY2-R.
