NPy (neuropeptide y) is a 36 amino acid protein that consists of a polyproline stretch followed by an amphipathic a-helix.
NPy shares a 50% amino acid homology with pancreatic polypeptide gene (PP).
NPy is expressed throughout the central and peripheral nervous system, particularly in the deep layers of the cortex and smaller cell bodies in the white matter.
NPy interacts with the y-receptor family of G protein-coupled receptors.
NPy interacts with NPy receptor y1 to increase corticotropin levels and decrease noradrenaline levels in the hypothalamus.
Through interactions in the hypothalus, NPy plays important roles in the regulation of energy balance by stimulating food intake.
NPy favors energy storage by increasing lipoprotein lipase activity in white adipose tissue.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NPY. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 61-110 of Human NPY.
