The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions.
To date, four Notch homologs have been identified in mammals and have been designated Notch 1, Notch 2, Notch 3 and Notch 4.
The Notch genes are expressed in a variety of tissues in both the embryonic and adult organism, suggesting that the genes are involved in multiple signaling pathways.
The Notch proteins have been found to be overexpressed or rearranged in human tumors.
Ligands for Notch include Jagged, Jagged2 and Delta.
Jagged can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes.
Jagged2 is thought to be involved in the development of various tissues whose development is dependent upon epithelial-mesenchymal interactions.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of Cleaved-Notch. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 1734-1800 of Human Notch 2.
