Nopp140, previously named p130, is a nucleolar phosphoprotein that has been shown to exist in multiple forms with different sizes.
Nopp140 functions both as a chaperone for import and/or export from the nucleolus and as a transcription factor.
Nopp140 was originally identified from rat liver as an NLS (nuclear localization signal)-binding protein, and has been further characterized as an RNAP (RNA Polymerase)-interacting protein.
Nopp140 also associates with the general transcription factor TFIIB, and the protein kinase casein kinase II (CKII).
CKII heavily phosphorylates Nopp140 to mediate binding of Nopp140 to NLS.
Nopp 140 colocalizes with another nucleolar protein, NAP57, in the nucleolus and coiled bodies, and is thought to be involved in activities carried out within the nucleolus.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NOLC1. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 654-699 of Human NOLC1.
