GADD 153, a growth arrest and DNA damage-inducible gene, encodes a C/EBP-related nuclear protein.
This protein has also been designated C/EBP-homologous protein (CHOP-10 or C/EBP zeta).
GADD 153 expression is induced by a variety of cellular stresses, inducing nutrient deprivation and metabolic perturbations.
GADD 153 functions to block cells in G1 to S phase during cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD 153 dimers away from “classical” C/EBP binding sites, recognizing instead unique “nonclassical” sites.
Thus, GADD 153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
GADD 153 belongs to the CBF/MAK21 family, which also includes NOC2L, NOC3L and NOC4L.
NOC2L is a 749 amino acid nuclear protein that may play a role in cell cycle regulation.
Applications:Suitable for use in Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.05% sodium azide, pH 7.2.
Specificity：Recognizes endogenous levels of NOC2L. Species Crossreactivity: Human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to amino acids 602-650 of Human NOC2L.
