The proccess of cellular respiration is carried out by integral inner mitochondrial membrane proteins that facilitate the harnessing of energy released by the oxidation of NADH.
NNT (Nicotinamide nucleotide transhydrogenase), also known as mitochondrial NAD(P) transhydrogenase or pyridine nucleotide transhydrogenase, is a 1086 amino acid multi-pass mitochondrial inner membrane protein.
NNT is a homodimer with an N-terminal section belonging to the AlaDH/PNT family and a C-terminal section belonging to the PNT beta subunit family.
NNT catalyzes the transfer of a hydride ion from NADH to NADP+ and functions as a mitochondrial inner membrane proton pump.
Using the energy of the proton gradient created by the electron transport chain, NNT produces high concentrations of NADPH, which is used in free radical detoxification and biosynthesis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid PBS, 0.1% sodium azide, 50% glycerol.
Specificity：Recognizes endogenous levels of NNT. Species Crossreactivity: Human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：A synthetic peptide corresponding to residues in Human NNT.
